Activation of protein kinase C results in down-modulation of different recombinant GABAA-channels

Modulation by palmitoylcarnitine of protein kinase C activation.

Palmitoylcarnitine, a reported protein kinase C inhibitor, enhanced the phorbol ester dependency of the enzyme, augmenting protein kinase C activity in the presence of phorbol esters such as phorbol 12,13-dibutyrate while inhibiting the basal activity measured in the presence of calcium plus phosphatidylserine. Weakly potent phorbol esters such as phorbol 12,13-diacetate and 4-O-methylphorbol 1...

Protein Kinase C Enhances Recombinant Bovine al ply21 GABAA Receptor WholeCell Currents Expressed in L929 Fibroblasts

The Bl and ~21 subunits of the y-aminobutyric acid type A receptor (CABAR) contain phosphorylation sites for PKC. To determine the effect of PKC on CABAR function, whole-cell recordings were obtained from mouse fibroblasts expressing recombinant alBly2L receptors, and catalytically active PKC (PKM) was applied via the recording pipette. The first experiment was a population study. Intracellular...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Activation of Protein Kinase C

GABAA receptor phosphorylation and functional modulation in cortical neurons by a protein kinase C-dependent pathway.

GABA(A) receptors are critical mediators of fast synaptic inhibition in the brain, and the predominant receptor subtype in the central nervous system is believed to be a pentamer composed of alpha, beta, and gamma subunits. Previous studies on recombinant receptors have shown that protein kinase C (PKC) and PKA directly phosphorylate intracellular serine residues within the receptor beta subuni...